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The antioxidant action of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-α]pyrazin-3-one (MCLA), a chemiluminescence probe to detect superoxide anions

✍ Scribed by Yoshiko Tampo; Maki Tsukamoto; Masanori Yonaha

Book ID
117109349
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
123 KB
Volume
430
Category
Article
ISSN
0014-5793

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## Abstract The chemiluminescence of the __Cypridina__ luciferin analogue, 2‐methyl‐6‐(p‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one (MCLA) was observed at 462nm in the presence of horseradish peroxidase (HRP) and the total spectrum of light emitted was found to depend linearly on HRP con

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The Cypridina luciferin analog, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA), in Hanks' balanced salt solution, emitted a weak luminescence which was not affected by superoxide dismutase or catalase and was not augmented by resting human granulocytes. In contrast, activated granulo