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Determination of horserdish peroxidase concentration using the chemiluminescence ofCypridina luciferin analogue, 2 methyl-6-(p-methyoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one

✍ Scribed by Mitani, Motohiro ;Yokoyama, Yuko ;Ichikawa, Syuji ;Sawada, Hideo ;Matsumoto, Takeo ;Fujimori, Ken ;Kosugi, Masanori

Publisher
John Wiley and Sons
Year
1994
Weight
379 KB
Volume
9
Category
Article
ISSN
0884-3996

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✦ Synopsis

Abstract

The chemiluminescence of the Cypridina luciferin analogue, 2‐methyl‐6‐(p‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐a]pyrazin‐3‐one (MCLA) was observed at 462nm in the presence of horseradish peroxidase (HRP) and the total spectrum of light emitted was found to depend linearly on HRP concentration. Methods for the determination of HRP concentration using the chemiluminescence was investigated. HRP could be detected in the range from 100 pmol/L to 100nmol/L under the optimum condition, H~2~O~2~ (10mmol/L) and MCLA (10μmol/L) at pH 5.8.

📜 SIMILAR VOLUMES

Measurement of rate constants for quench
Measurement of rate constants for quenching singlet oxygen with aCypridina luciferin analog (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo[1,2-a]pyrazin-3-one) and sodium azide
✍ Mashiko, Shinro ;Suzuki, Nobutaka ;Koga, Satoshi ;Nakano, Minoru ;Goto, Toshio ; 📂 Article 📅 1991 🏛 John Wiley and Sons ⚖ 347 KB 👁 1 views

## Abstract The rate constants for [^1^O~2~] [MCLA] and [^1^O~2~][NaN~3~] were measured by quenching the near‐infrared emission (^1^Δ~g~→^3^∑~__g__~) in steady state with MCLA and NaN~3~, respectively. ^1^O~2~ was constantly generated by energy transfer to O~2~ from Ar laser‐excited Rose Bengal. Th