The anaerobic life of Bacillus subtilis: Cloning of the genes encoding the respiratory nitrate reductase system
β Scribed by Tamara Hoffmann; Barbara Troup; Alexandra Szabo; Christoph Hungerer; Dieter Jahn
- Book ID
- 109320739
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 999 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0378-1097
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The reduced methylviolegen-nitrate reductase of the obligate thermophile, Bacillus stearothermophilus NCA strain 2 I84 (ATCC 120 16), has been purified to electrophoretic homogeneity, 53-fold with a yield of 12.5%. The purification procedure involved solubilization with octyl glucoside, ammonium sul
The ptsG gene of Bacillus subtilis encodes Enzyme IIGlc of the phosphoenolpyruvate: glucose phosphotransferase system. The 3' end of the gene was previously cloned and the encoded polypeptide found to resemble the Enzymes IIIGlc of Escherichia coli and Salmonella typhimurium. We report here cloning