Cloning of the glycerol kinase gene of Bacillus subtilis
โ Scribed by Christina Holmberg; Blanka Rutberg
- Book ID
- 109314677
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 338 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0378-1097
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The ptsG gene of Bacillus subtilis encodes Enzyme IIGlc of the phosphoenolpyruvate: glucose phosphotransferase system. The 3' end of the gene was previously cloned and the encoded polypeptide found to resemble the Enzymes IIIGlc of Escherichia coli and Salmonella typhimurium. We report here cloning
Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform