𝔖 Bobbio Scriptorium
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The amyloplast proteome of potato tuber

✍ Scribed by Allan Stensballe; Simon Hald; Guy Bauw; Andreas Blennow; Karen G. Welinder

Book ID
111312352
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
254 KB
Volume
275
Category
Article
ISSN
1432-1327

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Amyloplasts have been purified from potato tubers by mechanical homogenization and gravity sedimentation through Nycodenz. Based on the recovery and latency of organelle‐specific marker enzymes, the recovery of amyloplasts is approximately 13%, exhibiting 65% intactness, with less than 1% contaminat

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Amyloplasts have been isolated from tubers of potato plants (Solanum tuberosum, cv. Desir6e). As it is difficult to isolate amyloplasts that have a high starch content, we used transformed plants in which the content of starch was reduced. This was achieved by decreasing the activity of ADP-glucose