The Alkaline Elution Technique for Measuring DNA Single Strand Breaks: Increased Reliability and Sensitivity
β Scribed by C.J. Koch; A.R. Giandomenico
- Book ID
- 102965130
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 711 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0003-2697
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We reason that the failure to detect this type of DNA This paper reports a gravity-flow filter elution assay fragmentation in some model systems may be more that permits the simultaneous detection of DNA douindicative of the limited detection sensitivity of convenble strand breaks (DSBs, as neutral
A method is described for measuring the average number of nucleaseinduced single-and double-strand breaks per DNA molecule. The procedure involves measuring the weight-average molecular weight of DNase I-digested DNA under neutral and alkaline conditions. A statistical equation is used to calculate