The activity of PEG-modified chymotrypsin in aqueous and organic media
β Scribed by Carlo Pina; Douglas Clark; Harvey Blanch
- Book ID
- 105333209
- Publisher
- Springer-Verlag
- Year
- 1989
- Tongue
- English
- Weight
- 298 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0951-208X
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π SIMILAR VOLUMES
## Abstract Enzymes are attractive catalysts for the production of optically active compounds in organic solvents. However, their often low catalytic activity in such applications hampers their practical use. To overcome this, we investigated the effectiveness of the covalent modification of Ξ±βchym
Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, was modified with polyoxyethylene (PEG) or aerosol-OT (AOT), and the solubility, conformation, and catalytic activity of the modified subtilisins in some organic media were compared under the same conditions. The solubility of m