𝔖 Bobbio Scriptorium
✦   LIBER   ✦

THE ACTIVE CENTERS OF SERINE PROTEINASES

✍ Scribed by B. S. Hartley


Book ID
114880827
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
395 KB
Volume
227
Category
Article
ISSN
0890-6564

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Bovine trypsinogen and chymotrypsinogen were successfully refolded as the mixed disulfide of glutathione using cysteine as the disulfide interchange catalyst. The native structures were regenerated with yields of 40%-50% at pH 8.6 and 4"C, and the half-time for the refolding was approximately 60-75