The action of phospholipase C and lipase on black film bilayer membranes

✍ Scribed by H.Stewart Hendrickson; D.G. Rustad; E.M. Scattergood; D.E. Engle

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 367 KB
• Volume: 13
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(74)90042-5

✦ Synopsis

Phospholipase C(Q perfringens) added to one side ofa phosphatidyl eholine-phosphatidyl serine-cholesterol (0.7 : 0.3 : 1.0, w/w/w) bilayer membrane resulted in a rapid decrease in membrane resistance which leveled off after several minutes at about one-half its initial value. Pancreatic lipase added at this time resulted in a rapid increase in membrane resistance to a value somewhat higher than the initial. This effect was independent of the side to which the lipase was added, indicating that diglyceride is rapidly equilibrated across both sides of the membrane. When both phospholipase C and lipase were added at zero time to the same or opposite sides, the resistance decreased slightly and then increased to a value higher than the initial. Replacement of phosphatidyl choline by 2-hexadecoxy-3-octadecoxypropyl-phosphonylcholine, a phosphonate analog, resulted in an inhibition of the phospholipase C reaction which was equal to the percent analog. The analog, thus, seemed to act only as inert lipid and did not inhibit hydrolys of the active lipid.