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The action of lipase on chloroplast membranes: II. Polypeptide patterns of bean galactolipase- and phospholipase A2-treated thylakoid membranes

โœ Scribed by Z. Krupa


Publisher
Springer
Year
1983
Tongue
English
Weight
530 KB
Volume
4
Category
Article
ISSN
0166-8595

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โœฆ Synopsis


Thylakoid membranes obtained from bean chloroplasts treated with bean galactolipase or phospholipase A2 (from Crotalus terr. terr.) showed marked changes in their polypeptide patterns when separated on SDS-PAGE. The obtained results have been discussed with regard to the relationship between chloroplast lipids and polypeptides originating from chlorophyll-protein complexes of bean thylakoids. A coexistence between galactolipids and the peripheral antennae in PSI complex and LHCP 3 as well as a conspicuous role of phospholipids in PSI and PSII centre chloro-phyU-protein complexes has to be underlined.


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The action of lipases on chloroplast mem
โœ Z. Krupa ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Springer ๐ŸŒ English โš– 533 KB

Bean chloroplasts treated with galactolipase (lipolytic acyl hydrolase) isolated from bean leaves showed an inhibition of photosystem I activity as measured by methyl viologen-mediated oxygen uptake and NADP + photoreduction. This inhibition was partially reversed by exogenous plastocyanin added to