✦ LIBER ✦

The action of lipases on chloroplast membranesI. The release of plastocyanin from galactolipase-treated thylakoid membranes

✍ Scribed by Z. Krupa

Publisher: Springer
Year: 1982
Tongue: English
Weight: 533 KB
Volume: 3
Category: Article
ISSN: 0166-8595
DOI: 10.1007/bf00040707

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✦ Synopsis

Bean chloroplasts treated with galactolipase (lipolytic acyl hydrolase) isolated from bean leaves showed an inhibition of photosystem I activity as measured by methyl viologen-mediated oxygen uptake and NADP + photoreduction. This inhibition was partially reversed by exogenous plastocyanin added to galactolipase-treated thylakoid membranes. Galactolipase released substantial amounts of endogenous plastocyanin (about 40%) from bean chloroplasts. The results are discussed with regard to the localization of plastocyanin in thylakoid membranes.

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The action of lipase on chloroplast membranes: II. Polypeptide patterns of bean galactolipase- and phospholipase A2-treated thylakoid membranes

✍ Z. Krupa 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 530 KB

Thylakoid membranes obtained from bean chloroplasts treated with bean galactolipase or phospholipase A2 (from Crotalus terr. terr.) showed marked changes in their polypeptide patterns when separated on SDS-PAGE. The obtained results have been discussed with regard to the relationship between chlorop