Tet-system for the regulation of gene expression during embryonic development
โ Scribed by Lev M. Fedorov; Oleg Yu. Tyrsin; Veit Krenn; Elena V. Chernigovskaya; Ulf R. Rapp
- Book ID
- 110350835
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 250 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0962-8819
No coin nor oath required. For personal study only.
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## Abstract The amelogenins are the predominant matrix proteins in developing enamel and are crucial for proper enamel mineralization. Transgenic mice were constructed in order to identify the segment of the __amelogenin__ gene required for specific expression in enamel organ cells. A 3.5 kb fragme
## Background The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems are based on the Tet repressor (TetR) from transposon Tn__10__, a dimeric DNA-binding protein that binds to specific operator sequences (__tetO__). To allow