Tertiary Lymphoid Structures: Methods and Protocols

✍ Scribed by Marie-Caroline Dieu-Nosjean

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 292
• Series: Methods in Molecular Biology 1845
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications.

✦ Table of Contents

Front Matter ....Pages i-xii
Tertiary Lymphoid Structures Among the World of Noncanonical Ectopic Lymphoid Organizations (Aaron Silva-Sanchez, Troy D. Randall, Selene Meza-Perez)....Pages 1-15
Cellular and Vascular Components of Tertiary Lymphoid Structures (Christopher George Mueller, Saba Nayar, David Gardner, Francesca Barone)....Pages 17-30
Meningeal Immunity, Drainage, and Tertiary Lymphoid Structure Formation (Antoine Louveau)....Pages 31-45
Development of Tools for the Selective Visualization and Quantification of TLS-Immune Cells on Tissue Sections (Christophe Klein, Priyanka Devi-Marulkar, Marie-Caroline Dieu-Nosjean, Claire Germain)....Pages 47-69
A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures (Karīna Siliņa, Chiara Burkhardt, Ruben Casanova, Alex Solterman, Maries van den Broek)....Pages 71-86
Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer (Keith E. Steele, Charles Brown)....Pages 87-98
Defining High Endothelial Venules and Tertiary Lymphoid Structures in Cancer (Emma Jones, Awen Gallimore, Ann Ager)....Pages 99-118
Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure Using Laser Capture Microdissection (Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean, Jérémy Goc)....Pages 119-137
Quantifying Tertiary Lymphoid Structure-Associated Genes in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues (Chunyan Gu-Trantien, Soizic Garaud, Edoardo Migliori, Cinzia Solinas, Jean-Nicolas Lodewyckx, Karen Willard-Gallo)....Pages 139-157
Generation of Recombinant Monoclonal Antibodies from Single B Cells Isolated from Synovial Tissue of Rheumatoid Arthritis Patients (Elisa Corsiero, Lucas Jagemann, Michele Bombardieri, Costantino Pitzalis)....Pages 159-187
Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations (Priyanka Devi-Marulkar, Hélène Kaplon, Marie-Caroline Dieu-Nosjean, Myriam Lawand)....Pages 189-204
Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues (Coline Couillault, Claire Germain, Bertrand Dubois, Hélène Kaplon)....Pages 205-222
Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs (Jean-Luc Teillaud, Lucile Regard, Clémence Martin, Sophie Sibéril, Pierre-Régis Burgel)....Pages 223-239
Identification and Characterization of Tertiary Lymphoid Structures in Murine Melanoma (Anthony B. Rodriguez, J. David Peske, Victor H. Engelhard)....Pages 241-257
Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma (Kelli A. Connolly, Mursal Nader, Nikhil Joshi)....Pages 259-273
Targeting Tertiary Lymphoid Structures for Tumor Immunotherapy (Haidong Tang, Xiangyan Qiu, Casey Timmerman, Yang-Xin Fu)....Pages 275-286
Back Matter ....Pages 287-289

✦ Subjects

Biomedicine; Immunology