Lymphoma: Methods and Protocols

✍ Scribed by Ralf Küppers

Publisher: Springer New York;Humana Press
Year: 2019
Tongue: English
Leaves: 439
Series: Methods in Molecular Biology 1956
Edition: 2nd ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.

✦ Table of Contents

Front Matter ....Pages i-xii
Origin and Pathogenesis of B Cell Lymphomas (Marc Seifert, René Scholtysik, Ralf Küppers)....Pages 1-33
Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas (Emily Glynn, Lori Soma, David Wu, Brent L. Wood, Jonathan R. Fromm)....Pages 35-60
Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells (Ralf Küppers, Markus Schneider, Martin-Leo Hansmann)....Pages 61-75
PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations (Elke Boone, Kim C. Heezen, Patricia J. T. A. Groenen, Anton W. Langerak, On behalf of the EuroClonality Consortium)....Pages 77-103
Expression Cloning of Antibodies from Single Human B Cells (Hedda Wardemann, Christian E. Busse)....Pages 105-125
Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ-)PCR (Menno C. van Zelm)....Pages 127-138
Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas (Andreas Agathangelidis, Fotis Psomopoulos, Kostas Stamatopoulos)....Pages 139-155
Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies (Sebastian Böttcher)....Pages 157-197
MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR (Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H. J. van der Velden, Jacques J. M. van Dongen, Michael Kneba)....Pages 199-228
Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input (Dieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, Christoph Plass)....Pages 229-248
FISH and FICTION in Lymphoma Research (Maciej Giefing, Reiner Siebert)....Pages 249-267
NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma (Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, Agnieszka Dzikiewicz-Krawczyk)....Pages 269-282
RNA Sequencing in B-Cell Lymphomas (Da Wei Huang, Moez Dawood, Calvin A. Johnson, Roland Schmitz)....Pages 283-303
Studying Cancer Heterogeneity by Single-Cell RNA Sequencing (Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, Wolfgang Enard)....Pages 305-319
Evaluation of Whole Genome Sequencing Data (Daniel Hübschmann, Matthias Schlesner)....Pages 321-336
Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines (Daniel E. Webster, Sandrine Roulland, James D. Phelan)....Pages 337-350
Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens (Katarzyna Tomska, Sebastian Scheinost, Thorsten Zenz)....Pages 351-362
Proximity Ligation Assay (Ryan M. Young)....Pages 363-370
Phosphoproteomic Analysis of Signaling Pathways in Lymphomas (Björn Häupl, Henning Urlaub, Thomas Oellerich)....Pages 371-381
Ultrasensitive Detection of Circulating Tumor DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries (Miguel Alcaide, Christopher Rushton, Ryan D. Morin)....Pages 383-435
Back Matter ....Pages 437-439

✦ Subjects

Biomedicine; Cancer Research

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