𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Tentative identification of new metabolites of epimedin C by liquid chromatography–mass spectrometry

✍ Scribed by Minyan Liu; Shaohua Zhao; Zongquan Wang; Hongtao Wang; Xiaowei Shi; Ziming Lü; Honghui Xu; Hairong Wang; Yingfeng Du; Lantong Zhang

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
253 KB
Volume
34
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Epimedin C is one of the major bioactive constituents of Herba Epimedii__.__ The aim of this study is to characterize and elucidate the structure of metabolites in the rat after administration of epimedin C. Metabolite identification was performed using a predictive multiple reaction monitoring–information dependent acquisition–enhanced product ion (pMRM‐IDA‐EPI) scan in positive ion mode on a hybrid triple quadrupole‐linear ion trap mass spectrometer. A total of 18 metabolites were characterized by the changes in their protonated molecular masses, their MS/MS spectrum and their retention times compared with those of the parent drug. The results reveal possible metabolite profiles of epimedin C in rats; the metabolic pathways including hydrolysis, hydroxylation, dehydrogenation, demethylation and conjugation with glucuronic acid and different sugars were observed. This study provides a practical approach for rapidly identifying complicated metabolites, a methodology that could be widely applied for the structural characterization of metabolites of other compounds.

📜 SIMILAR VOLUMES

The structural identification of drug me
The structural identification of drug metabolites using thermospray liquid chromatography/mass spectrometry
✍ Iain G. Beattie; Timothy J. A. Blake 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB 👁 2 views

The structural identification of drug metabolites has been carried out using thermospray liquid chromatography/mass spectrometry (LC/MS). It has allowed the direct analysis of biological samples, in this case in vitro hepatocyte incubations, with the minimum of sample preparation. The technique also

Identification of metabolites of hexazin
Identification of metabolites of hexazinone by mass spectrometry
✍ Robert W. Reiser; Irvin J. Belasco; Robert C. Rhodes 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 428 KB 👁 1 views

The metabolites of hexazinone [3-cyclohe~yl-6-(dimethylamino)-l-methyl-1,3,5-triazine-Z,4(1H,3H)-dione] obtained in the rat and in plants were identified by mass spectrometry. Rat urine metabolites were identified from direct probe spectra obtained on metabolites separated by thin-layer chromatograp

Elucidation of urinary metabolites of fl
Elucidation of urinary metabolites of fluoxymesterone by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry
✍ Oscar J. Pozo; Wim Van Thuyne; Koen Deventer; Peter Van Eenoo; Frans T. Delbeke 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 340 KB

## Abstract The suitability of liquid chromatography tandem mass spectrometry (LC‐MS/MS) and gas chromatography mass spectrometry (GC‐MS) for the elucidation of fluoxymesterone metabolism has been evaluated. Electrospray ionization (ESI) and collision induced dissociation (CID) fragmentation in LC‐

Identification of drug metabolites in bi
Identification of drug metabolites in biological matrices by intelligent automated liquid chromatography/ tandem mass spectrometry
✍ Linda L. Lopez; Xiao Yu; Donghui Cui; Margaret R. Davis 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 102 KB 👁 2 views

A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography -tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates i