Tensammetric and indirect amperometric detection of phospholipids

✍ Scribed by Thomas Schmidt; Hendrik Emons

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 790 KB
• Volume: 3
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140030615

✦ Synopsis

Electrochemical detection procedures for electroinactive phospholipids were developed for flow injection (FI) systems and high performance liquid chromatography (HPLC). The methods are based on the adsorption of these surface-active compounds, which was characterized in solutions containing acetonitrile, water, and methanol. Tensammetric FI detection at mercury electrodes was systematically studied with respect to various experimental parameters. The method was also applied to the normal phase HPLC determination of phospholipids, but with higher detection limits. In addition, an indirect amperometric detection method based on the inhibition effect of adsorbed phospholipids on electrode reactions at glassy carbon fibers was explored. This approach can be also used for FI systems and in HPLC. Significantly wide linear dynamic ranges were obtained for the determination of phospholipids.