Temporary culture in isoleucine-free medium enhances transformation of 10T½ cells by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)
✍ Scribed by Joe W. Grisham; Diane S. Greenberg; Gary J. Smith; David G. Kaufman
- Book ID
- 119406608
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 304 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0006-291X
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The sensitivity to sister chromatid exchange (SCE) induction by N-methyl-nitro-N'-nitrosoguanidine (MNNG) in human, mouse, Chinese hamster, and Syrian hamster normal cell strains and in permanent transformed cell lines of the same species was compared. Exponentially growing or growth-inhibited cultu
## Abstract A cell line derived from a normal beagle embryo was treated __in vitro__ with various levels of N‐methyl‐N′‐nitro‐N‐nitrosoguanidine or dimethyl sulfoxide (control). Cells treated only with the carcinogen underwent morphologic alteration __in vitro__, and one of these altered cell lines