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Temporary chromosome number stabilization in potato cell cultures

✍ Scribed by Ursula Dathe; Günter Wersuhn

Publisher
Springer
Year
1990
Tongue
English
Weight
369 KB
Volume
22
Category
Article
ISSN
0167-6857

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✦ Synopsis

Cell cultures of Solanum chacoense (monohaploid) and Solanum tuberosum (tetraploid cultivars and parthenogenetically derived dihaploid clones) were found to be highly mixoploid.

Relative stabilization of chromosome number at the ploidy level of the original plant material was achieved in microcalli obtained from single cells or small cell colonies (up to about 5 cells) of stock callus lines. This relative stabilization was maintained over three subcultures, which is sufficient for selection procedures. It has been shown that the stabilization can be maintained during a number of further subcultures. Division centers were repeatedly observed in calli characterized by high mitotic activity. As has been shown for the first time there exist significant differences in the ploidy levels of several division centers within one and the same callus. This is of particular importance to callus subculture.

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## Abstract Cytogenetic analysis revealed low chromosome number, telomeric association, and pulverisation of chromosomes in three chromophobe renal cell carcinomas. One fully karyotyped and a previously published case showed the common loss of chromosomes 1, 2, 6, 10, 13, 17, and 21.