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Template properties of human dna-dependent rna polymerase II

✍ Scribed by Garcia, Hector D. ;Tsai, Ming-Jer ;Saunders, Grady F.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
541 KB
Volume
2
Category
Article
ISSN
0098-1532

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✦ Synopsis

Abstract

DNA‐dependent RNA polymerase II has been purified from lymphocytes of patients with chronic lymphocytic leukemia (CLL). Form II polymerase and Escherichia coli RNA polymerase have been used to study the transcription of human DNA and chromatin. The hybridization kinetics of the transcripts of DNA and chromatin by the human polymerase are quite different; chromatin transcripts hybridized to DNA at a much slower rate than DNA transcripts, whereas the transcripts of DNA and chromatin by bacterial polymerase have similar hybridization kinetics.

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