✦ LIBER ✦
Templates for eukaryotic RNA polymerase II: Artefacts can produce an apparent preference for denatured DNA over native DNA
✍ Scribed by William S. Dynan; Jerome J. Jendrisak; Richard R. Burgess
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 561 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Three artefacts have been identified which can reverse the preference of wheat germ RNA Polymerase II for native over heat-denatured calf thymus DNA. Selfabsorption during liquid scintillation counting, contamination of DNA with RNase. and the use of UTP-limiting substrate containing less UTP than ATP, CTP, or GTP all decrease measured enzyme activity on native DNA.,Eliminating these effects causes a 20-fold increase in the ratio of synthesis on native DNA to synthesis on heat-denatured DNA.