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Temperature-Sensitive Mutants of Escherichia coli with Defects in the Assembly of RNA Polymerase in vitro

✍ Scribed by Gerhard C. GROSS; Dorothy A. FIELDS; Ekkehard K. F. BAUTZ


Book ID
115116374
Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
476 KB
Volume
81
Category
Article
ISSN
1432-1327

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A cold-sensitive Ξ² subunit mutant RNA po
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A cold-sensitive mutation in the rpoB gene for the RNA polymerase beta subunit increasing the temperature of promoter opening on T2 phage DNA was obtained in Escherichia coli. The mutation also affects the stages preceding promoter opening by increasing the dissociation rate of RNA polymerase--DNA c