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Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12

✍ Scribed by Bohman, Kaj ;Isaksson, Leif A.


Publisher
Springer
Year
1979
Tongue
English
Weight
173 KB
Volume
176
Category
Article
ISSN
0026-8925

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✦ Synopsis


Two mutants with a defective cysteinyl-tRNA synthetase have been found in a collection of spontaneous temperature sensitive mutants. The mutated gene, which is designated cysS, is closely cotransduced with purE.


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Mutations in the tryptophanyl-transfer r
✍ Bohman, Kaj ;Isaksson, Leif A. πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 394 KB

Strains of Escherichia coli K-12 with altered tryptophanyl-tRNA ligases, conferring temperature-sensitivity for growth, have been isolated as spontaneous one-step mutants. The mutated enzymes differ markedly in activity from the wildtype, even at the permissive temperature. When assayed at the non-p