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A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12

โœ Scribed by Bohman, Kaj ;Isaksson, Leif A.


Publisher
Springer
Year
1980
Tongue
English
Weight
207 KB
Volume
177
Category
Article
ISSN
0026-8925

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๐Ÿ“œ SIMILAR VOLUMES


Temperature-sensitive mutants in cystein
โœ Bohman, Kaj ;Isaksson, Leif A. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 173 KB

Two mutants with a defective cysteinyl-tRNA synthetase have been found in a collection of spontaneous temperature sensitive mutants. The mutated gene, which is designated cysS, is closely cotransduced with purE.

Tn1 insertion mutagenesis in Escherichia
โœ Harayama, Shigeaki ;Tsuda, Masataka ;Iino, Tetsuo ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 376 KB

A method for Tn1 insertion mutagenesis in Escherichia coli has been developed using pTH10, a mutant plasmid of RP4 temperature-sensitive for maintenance. The mutagenesis involves three steps. Firstly, from strains carrying pTH10 showing resistance to the antibiotics kanamycin, tetracycline, and ampi