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Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii

✍ Scribed by Cross, John ;McMahon, Daniel


Book ID
104693078
Publisher
Springer
Year
1976
Tongue
English
Weight
725 KB
Volume
147
Category
Article
ISSN
0026-8925

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✦ Synopsis


Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.


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