## Abstract The __in vivo__ biologic activities of a clonal isolate of the Rauscher strain of murine leukemia virus and several conditional lethal mutants with impaired replicative functions at the nonβpermissive temperature were investigated. The parental virus induced a rapidly developing lymphoi
Temperature-sensitive breakdown in vivo of polysomes with mutants of Chlamydomonas reinhardii
β Scribed by Cross, John ;McMahon, Daniel
- Book ID
- 104693078
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 725 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Two temperature-sensitive mutants of Chlamydomonas reinhardii Dangeard which are defective in protein synthesis were examined. Both show breakdown of their polysomes at the restrictive temperature into monosomes which do not contain fragments of mRNA. Many of the ribosomes still contain nascent peptides able to react with puromycin. The polysome breakdown involves only cytoplasmic (80S) ribosomes and is prevented or reversed when ribosome translocation is inhibited with cyloheximide.
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