Chinese hamster fibroblasts in monolayer cultures were synchronized by accumulating mitotic cells in the presence of Colcemid, removing the mitotic cells with a brief trypsin treatment, and growing them in medium lacking Colcemid. Such cultures grew normally and exhibited no significant deviations f
Temperature limits of mitosis in mammalian cell cultures
โ Scribed by F. V. Sushkov; V. V. Portugalov
- Publisher
- Springer US
- Year
- 1975
- Tongue
- English
- Weight
- 227 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0007-4888
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract We sought a cultured cell line with Proline Oxidase activity to study the regulation and physiologic role of the enzyme in mammalian tissues. Among the cell lines tested, only LLCโRK~1~ cells, derived from rabbit kidney, had significant Proline Oxidase activity; the K~m~ for proline of
Apoptosis is now recognized as a significant problem in mammalian cell culture. Therefore, in this study, a single gene and multigene approach to inhibit apoptosis has been examined. Stable Chinese hamster ovary (CHO) cell lines were generated to overexpress different single, dual, and triple combin