✦ LIBER ✦

Temperature gradient gel electrophoresis for analysis of clonal evolution in non-Hodgkin's lymphoma of the thyroid

✍ Scribed by Markus Tiemann; Martin A. O. H. Menke; Robert Asbeck; Hans-Heinrich Wacker; Reza Parwaresch

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 462 KB
Volume: 16
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501601118

No coin nor oath required. For personal study only.

✦ Synopsis

To analyze the transition of an autoimmune disease into a mucosa-associated lymphoid tissue-non-Hodgkin's lymphoma (MALT-NHL), we investigated a total of 27 cases of clinically diagnosed autoimmune thyroiditis with lymphoid hyperplasia. Three cases of thyroid hyperplasia served as controls. Monoclonal B cells were detected by studying rearrangement patterns of the hypervariable CDR I11 regions within the immunoglobulin heavy chain gene locus and the T-cell receptor y chain gene (TCRG). We used a seminested polymerase chain reaction (PCR) to demonstrate immunoglobulin rearrangements and a multiplex PCR for TCRG rearrangements. The PCR products were analyzed by temperature gradient gel electrophoresis to expand mixtures of homo-and heteroduplices within heterogeneous populations of B cells. With this approach we found monoclonality in 14 of the 27 cases of Hashimoto's disease. In a reinvestigation we discovered additional histological and immunohistochemical features of MALT-NHL in 17 cases. The 14 cases of thyroiditis with clonally expanded B cells clearly demonstrate the transition from autoimmune disease to non-Hodgkin's lymphoma.

📜 SIMILAR VOLUMES

An approach to the sensitivity of temper

An approach to the sensitivity of temperature-gradient gel electrophoresis in the detection of clonally expanded T-cells in cutaneous T-cell lymphoma

✍ Cordula Ahnhudt; J. Marcus Muche; Katharina Dijkstal; Wolfram Sterry; Ansgar Luk 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 456 KB 👁 2 views

Detection of T-cell clonality by polymerase chain reaction (PCR) and high-resolution electrophoresis facilitates differentiation of early stages of cutaneous T-cell lymphoma (CTCL) from benign T-cell-rich dermatoses. However, data regarding the sensitivity of the various electrophoresis techniques d

Analysis of the NF1 gene by temperature

Analysis of the NF1 gene by temperature gradient gel electrophoresis reveals a high incidence of mutations in exon 4b

✍ Mohammed R. Toliat; Fikret Erdogan; Andreas Gewies; Raimund Fahsold; Annegret Bu 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 116 KB 👁 2 views

APC mutations in human colorectal adenom

APC mutations in human colorectal adenomas: analysis of the mutation cluster region with temperature gradient gel electrophoresis and clinicopathological features

✍ Mulkens, Judith; Poncin, Jacques; Arends, Jan Willem; de Goeij, Anton F. P. M. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 194 KB 👁 2 views

To date, the earliest mutations in colorectal adenomas have been found in the adenomatous polyposis coli (APC) gene, considered the 'gatekeeper' in tumourigenesis. To study the types of APC gene mutations and their relation to clinicopathological features which are associated with malignant potentia

Establishment of experimental conditions

Establishment of experimental conditions for the rapid detection of mutations in the von Hippel-Lindau gene by parallel temperature gradient gel electrophoresis

✍ Ana Hernández; Alexander Meyer; Jürgen Enczmann; Rolf Ackermann; Peter Wernet 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 94 KB 👁 2 views

Agreement rates and American-Japanese pa

Agreement rates and American-Japanese pathologists' comparability of a modified working formulation for non-Hodgkin's lymphomas an analysis of the cases collected for the fifth international workshop on chromosomes in leukemia-lymphoma

✍ Koji Nanba; Hisashi Yamamoto; Nanao Kamada; Masahiro Kikuchi; Taizan Suchi; Glau 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 709 KB