## Detection of clonal T cells in cutaneous T cell lymphoma by polymerase chain reaction: Comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels Cutaneous T cell lymphomas (CTCL) can be diff
✦ LIBER ✦
Temperature gradient gel electrophoresis for analysis of a polymerase chain reaction-based diagnostic clonality assay in the early stages of cutaneous T-cell lymphomas
✍ Scribed by Martin A. O. H. Menke; Markus Tiemann; Dörthe Vogelsang; Christina Boie; Reza Parwaresch
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 604 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
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Using Southern blotting for the diagnosis of clonality in peripheral T-cell lymphomas (PTCLs), analysis of the T-cell receptor (TCR) y gene rearrangement was shown to be more informative than that of the TCR /? gene rearrangement. In order to amplify every VJy rearrangement, a polymerase chain react