VJ REARRANGEMENTS OF THE TCRγ LOCUS IN PERIPHERAL T-CELL LYMPHOMAS: ANALYSIS BY POLYMERASE CHAIN REACTION AND DENATURING GRADIENT GEL ELECTROPHORESIS
✍ Scribed by THEODOROU, IOANNIS; BIGORGNE, CLAUDE; DELFAU, MARIE-HÉLÈNE; LAHET, CHANTAL; COCHET, GILLES; VIDAUD, MICHEL; RAPHAEL, MARTINE; GAULARD, PHILIPPE; FARCET, JEAN-PIERRE
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 771 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
Using Southern blotting for the diagnosis of clonality in peripheral T-cell lymphomas (PTCLs), analysis of the T-cell receptor (TCR) y gene rearrangement was shown to be more informative than that of the TCR /? gene rearrangement. In order to amplify every VJy rearrangement, a polymerase chain reaction (PCR) procedure using newly designed GC-clamp primers has been developed. All primers can be mixed in a single multiplex PCR. PCR products are analysed by denaturing gradient gel electrophoresis (DGGE), providing tumour-specific imprints inasmuch as the procedure characterizes N sequence polymorphism at the V J junctions. In a series of 30 PTCL cases, the PCR procedure demonstrated 27 cases to be clonally rearranged and failed in three cases. PCR was more accurate than Southern blotting, showing 47 rearranged y alleles, four of which were undetectable on the Southern blot. When lymphomas were studied a t different sites and at relapse, the DGGE pattern remained unchanged. In PTCL, the proposed PCR is helpful for the diagnosis and staging of the disease and should improve the follow-up monitoring. The undetectability of clonal rearrangements in a few cases is discussed in the light of concepts of lymphomagenesis and T-cell differentiation.