Temperature dependency of induction of sexual agglutinability by α pheromone in the yeastSaccharomyces cerevisiae

✍ Scribed by Kayoko Nishi; Naohiko Yanagishima

• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 453 KB
• Volume: 132
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00407957

✦ Synopsis

When c~ pheromone-pretreated cells of an inducible a strain of Saccharomyces cerevisiae carrying the inducible gene saal were incubated in a growth medium at 28~ induction of sexual agglutinability began after a 10 min lag period. If the cells were incubated at 38~ during the lag period, no induction occurred even after incubation at 28 ~ C. Contrary to this, if the cells were incubated at 28 ~ C during the lag period, almost complete induction occurred, even after transfer to 38 ~ C. Temperature shift experiments revealed that 5 rain incubation at 28~ was necessary for the initiation of the temperature-sensitive period and further 5 rain incubation for the completion of the period. The temperature-sensitive period was sensitive to phenylmethylsulfonyl fluoride.