When c~ pheromone-pretreated cells of an inducible a strain of Saccharomyces cerevisiae carrying the inducible gene saal were incubated in a growth medium at 28~ induction of sexual agglutinability began after a 10 min lag period. If the cells were incubated at 38~ during the lag period, no inductio
Genetic analysis of inducible sexual agglutination ability in the yeastSaccharomyces cerevisiae
โ Scribed by Yoshiyuki Nakagawa
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 423 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0302-8933
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โฆ Synopsis
Genetic regulation of the inducibility of sexual agglutination ability in the yeast Saccharomyces cerevisiae was studied. Detailed analysis of the degree of sexual agglutination was carried out; it showed that a greater number of genes are involved in the regulation of inducible sexual agglutination in strain HI-0 than previously assumed. Although dominancy of inducible phenotype over constitutive was confirmed, the effectiveness of one gene changing the constitutive phenotype to the inducible seemed to be somewhat low. Quantity per cell of agglutination substances responsible for sexual agglutination increased as the agglutination ability became greater.
๐ SIMILAR VOLUMES
We have identified a recessive a-mating-typespecific gene agal causing agglutinabflity defect without significant effects on other sexual activities, a cells carrying agal showed sexual agglutination with a cells but a cells carrying agal showed sexual agglutination with neither a cells nor a cells,
Five mutants carrying antisuppressors which reduce the efficiency of SUP46 suppressor of the yeast, Saccharomyces cerevisiae, were isolated. One of them was found to have an altered protein, S27, of 40S ribosomal subunit by using two-dimensional gel electrophoresis. A single recessive gene, designat