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Temperature dependence of the survival of human erythrocytes frozen slowly in various concentrations of glycerol

โœ Scribed by Souzu, H.; Mazur, P.


Book ID
118517553
Publisher
Biophysical Society
Year
1978
Tongue
English
Weight
614 KB
Volume
23
Category
Article
ISSN
0006-3495

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79ยฐC before direct transfer to liquid nitrogen (-196ยฐC) and thawed slowly ( -20"C/min) or rapidly ( -500ยฐC/min). T