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Effect of Osmolality and Oxygen Tension on the Survival of Mouse Sperm Frozen to Various Temperatures in Various Concentrations of Glycerol and Raffinose

✍ Scribed by Chihiro Koshimoto; Edna Gamliel; Peter Mazur


Book ID
115595671
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
186 KB
Volume
41
Category
Article
ISSN
0011-2240

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## Abstract Frozen mouse eggs were examined to determine the effects of low temperatures, concentration of cryoprotective agents and cooling rates on their survival, fertilizability in vitro and subsequent development. Dimethyl sulfoxide administered at 1.5 M concentration was found to be the most