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Telomerase activity in benign and malignant cytologic fluids

✍ Scribed by Mu, Xiao C. ;Brien, Thomas P. ;Ross, Jeffrey S. ;Lowry, Charles V. ;McKenna, Barbara J.

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
240 KB
Volume
87
Category
Article
ISSN
0008-543X

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✦ Synopsis

BACKGROUND.

Telomerase is a ribonucleoprotein that maintains telomeric base pair repeats at the ends of mammalian chromosomes during DNA replication.

Telomerase is expressed in various human tumors, some normal tissues, and immortalized cell lines. The assay of telomerase activity has potential as an adjunct for cancer detection in cytologic fluids.

METHODS.

Twenty-four unfixed cytologic fluids, including 13 ascitic fluids, 7 pleural fluids, 3 pelvic washings, and 1 bronchial washing, were prepared for polymerase chain reaction (PCR)Οͺbased telomeric repeat amplification protocol (Oncor, Inc., Gaithersburg, MD). Telomerase activity was determined by PCR. The presence of a ladder of products with 6 base pair increments, separated by polyacrylamide gel electrophoresis and detected by phosphoimaging, was considered a positive result. Results were compared with cytologic evaluation of alcohol fixed, Papanicolaou stained smears.

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