Technology platforms for 3D cell culture : a user's guide

✍ Scribed by Przyborski, Stefan

• Publisher: Wiley/Blackwell
• Year: 2017
• Tongue: English
• Leaves: 416
• Category: Library

✦ Synopsis

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. -Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture -Features contributions from leading developers and researchers active in 3D cell technology -Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied -Includes information on resources and technical support to help initiate the use of 3D culture methods. Read more...

Abstract:

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Read more...

✦ Table of Contents

Content: Title Page
Copyright Page
Contents
List of contributors
Preface
List of abbreviations
Chapter 1 An introduction to the third dimension for routine cell culture
Introduction
Aggregate-based technologies
Scaffold-based technologies
3D bioreactors
Barriers to adoption and future directions
References
Part I Aggregate-based technologies
Chapter 2 Gravity-enforced microtissue engineering
Introduction
Product description
Tumour microtissue size profiling for drug testing
Conclusions
Troubleshooting and notes (Table 2.3)
Summary
References. Chapter 3 Physiologically relevant spheroid models for three-dimensional cell culture Introduction
Description of technology
Experimental results obtained using the Perfecta3D hanging drop plates
Troubleshooting
Conclusion
References
Chapter 4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture
Introduction
Description of technology
Example of application
Troubleshooting
Summary
References
Chapter 5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues --
the 3D Petri Dish® IntroductionDescription of technology
Applications
Protocols
Troubleshooting
Conclusion
References
Chapter 6 Organotypic microtissues on an air-liquid interface
Introduction
Description of technology
3D neural OrganDOT" tissues for neurotoxicology studies
Troubleshooting
References
Part II Hydrogels
Chapter 7 Materials and assay systems used for three?dimensional cell culture
Introduction
Description of technology
Applications of 3D cultures in vitro
Conclusion
References
Chapter 8 HyStem®, a customisable hyaluronan?based hydrogel matrix for 3D cell culture
Introduction. Description of technologyApplications
Troubleshooting
Conclusion
Appendix
References
Chapter 9 3-D Life biomimetic hydrogels: A modular system for cell environment design
Introduction
Applications
Description of technology
A tumour-stroma model: 3D co-culture of MCF-7 cells and primary fibroblasts
Troubleshooting
Conclusion
References
Part III Scaffolds
Chapter 10 Alvetex®, a highly porous polystyrene scaffold for routine three-dimensional cell culture
Introduction
Description of technology
Example applications of Alvetex Scaffold technology
Troubleshooting
Conclusion.

✦ Subjects

Cell culture;Cytology -- Technique;SCIENCE -- Life Sciences -- Anatomy & Physiology;Cell Culture Techniques -- methods;Tissue Engineering -- methods;Tissue Scaffolds;Hydrogels