Technologies for large-scale physical mapping of human chromosomes

✍ Scribed by Tony J. Beugelsdijk

• Book ID: 104424829
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 673 KB
• Volume: 31
• Category: Article
• ISSN: 1381-141X
• DOI: 10.1016/s1381-141x(96)80005-0

✦ Synopsis

Developing and characterization ordered clone collection from human chromosome specific DNA libraries is proceeding as part of a larger effort to construct a physical map of the entire human genome. The robotics and automation section at Los Alamos has been focussed on developing the hardware and software tools required to support this objective.

'Ikese tools are typically integrated systems that combine an intuitive user interface, a database, as well as the relevant hardware technologies. To date, we have developed a system to automatically grid clones onto nylon filters in high density arrays. We have also developed a hybridization autoradiograph software scoring tool that combines image analysis, databasing, and a user interface.