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Technique for injection into the sciatic nerve of the mouse for quantitative in vivo metabolic studies

✍ Scribed by Anthony M. Heape; Françoise Boiron; Claude Cassagne

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
296 KB
Volume
155
Category
Article
ISSN
0003-2697

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✦ Synopsis

In this paper we describe a technique for intraneural injections, applicable to mouse peripheral nerves, which, compared with previous techniques, reduces trauma to the nerves and increases the level and reduces the variability of label recovery. Our technique employs glass needles (tip diameter, 50 micron) linked to a peristaltic pump by polyethylene tubing to inject small volumes (in the microliter range) of radiolabeled substrate solutions into mouse sciatic nerves, and allows the recovery of 20.9 +/- 1.9% (mean +/- standard deviation) and 30.5 +/- 4.8% of the injected radioactivity for 2 microliter [3H]acetate and 0.5 microliter of [3H]stearate, respectively.

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