Targeting Vectors for Gene Diversification by Meiotic Recombination in Neurospora crassa
β Scribed by J.Paul Rasmussen; Frederick J. Bowring; P.Jane Yeadon; David E.A. Catcheside
- Book ID
- 112263612
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 216 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0147-619X
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π SIMILAR VOLUMES
Recombination at his-3 in Neurospora crassa is thought to be initiated through a site designated cog which lies in the his-3 to ad-3 interval of linkage group I. Fragments of the his-3 gene were used to transform various his-3 mutant alleles to prototrophy in order to link the genetic map to the nuc
A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'