Targeting of Single-Stranded Oligonucleotides through Metal-Induced Cyclization of Short Complementary Strands

✍ Scribed by Fabrice Freville; Tristan Richard; Katell Bathany; Serge Moreau

• Book ID: 102253837
• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: German
• Weight: 276 KB
• Volume: 89
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.200690265

✦ Synopsis

Abstract

A new strategy to cyclize short synthetic oligonucleotides on DNA or RNA target strands is described. The approach is based on metal‐templated cyclization of short synthetic oligonucleotides conjugated with two chelating 2,2′ : 6′,2′′‐terpyridine (Tpy) moieties at their 3′‐ and 5′‐ends. Cyclization after metal addition (Zn^2+^, Fe^2+^) was demonstrated by means of thermal‐denaturation experiments, MALDI‐Q‐TOF‐MS, and gel electrophoresis (PAGE). 1D‐ and 2D‐NMR Experiments were performed to analyze the association of complementary strands after metal‐mediated cyclization. Our protocol allows the efficient circularization of synthetic oligonucleotides. Thereby, the hybridization on a complementary strand was more efficient with an RNA target strand and a 2′‐O‐methylated circularized oligomer.