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Targeting Carbonic Anhydrases with Fluorescent BODIPY-Labelled Sulfonamides

✍ Scribed by Philip A. Waghorn; Michael W. Jones; Alan McIntyre; Alessio Innocenti; Daniela Vullo; Adrian L. Harris; Claudiu T. Supuran; Jonathan R. Dilworth

Book ID
112082349
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
668 KB
Volume
2012
Category
Article
ISSN
1434-1948

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## Abstract A practical laboratory experiment is described that illustrates the application of fluorescence resonance energy transfer to the study of protein‐ligand binding. The affinities of wild‐type and mutant human carbonic anhydrase II for dansylamide were determined by monitoring the increase