𝔖 Bobbio Scriptorium
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Targeted liposomes: A method for preparation and analysis

✍ Scribed by Vilma K. Jansons; Patricia L. Mallett

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
499 KB
Volume
111
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple method for the preparation of antibody targeted iiposomes is described. It consists of the covalent coupling of the F(ab'), fragment of antibody to phosphatidylethanolamine and subsequent association of this complex with dimyristoylphosphatidylcholine liposomes. The side effects during the coupling are minimized with prior citraconylation. The association of the protein with the liposomes is demonstrated by gel filtration. Binding assays with antigen-coated Staphyloccus aureus are used to evalute the immunologically specific recognition and the membrane stability of the targeted liposomes.

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