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A method to measure critical detergent parameters. Preparation of liposomes

✍ Scribed by J. Lasch; V.R. Berdichevsky; V.P. Torchilin; Regine Koelsch; K. Kretschmer

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
430 KB
Volume
133
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple physical method to determine the monomer concentration of detergents below as well as above the critical micelle concentration based on the bubble-pressure measurement is described. Aggregated surfactant molecules (micelles) and phospholipid vesicles if present in the sample will not disturb the measurements. Three applications of the method relevant to the preparation of liposomes arc shown: (i) measurements of critical micelle concentrations, (ii) evaluation of the affinity constant of the interaction of detergents with liposomal membranes, and (iii) monitoring of residual detergent in liposome preparations during dialysis or after gel chromatography of mixed micellederived liposomes. It was found that the efficiency of detergents to produce liposomes during their removal depends on their critical micelle concentrations as well as on their affinity to liposomal membranes.

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