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Target factor analysis: UV spectrophotometry for the simultaneous determination of six amino acids

✍ Scribed by Pan Zhongxiao; Xia Siqing; Si Shengzhu; Zhang Maosen; Shi Leming; Liu Xinan

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 389 KB
Volume: 4
Category: Article
ISSN: 0886-9383
DOI: 10.1002/cem.1180040406

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✦ Synopsis

The UV spectra of mixture solutions consisting of tyrosine, tryptophane, phenylalanine, cystine, histidine and 3,4-dihydroxyl phenylalanine have been measured. The numbers, identities and concentrations of the amino acids in the mixtures have been determined successfully using target factor analysis. The effects of the wavelength range and the selected sampling interval on the results are discussed. Twenty-five synthetic mixture samples have been analysed successfully. The average recoveries are 98-9 for Tyr, 96'5 for Trp, 105.6 for Phe, 98.1 for Cys, 98.9 for His and 106.4 for Di-phe. The results obtained are in good agreement with those obtained by the Kalman filter method.

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