TAP1-independent loading of class I molecules by exogenous viral proteins

✍ Scribed by Martin F. Bachmann; Annette Oxenius; Hanspeter Pircher; Hans Hengartner; Philip A. Ashton-Richardt; Susumu Tonegawa; Rolf M. Zinkernagel

• Book ID: 102828150
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 518 KB
• Volume: 25
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830250637

✦ Synopsis

TAP1-independent loading of class I molecules by exogenous viral proteins

Presentation of peptides derived from endogenous proteins on class I molecules needs functional TAP peptide transporters. To reveal whether class I-associated presentation of exogenous proteins also required the presence of TAP transporters, we assessed in vitro the ability of spleen cells and macrophages from TAP1deficient mice (TAP1 -/-) to present peptides derived from exogenous recombinant viral proteins on their class I molecules. We found that recombinant glycoand nucleoprotein from lymphocytic choriomeningitis virus and nucleoprotein of vesicular stomatitis virus were presented as efficiently by TAPl-/cells as by control cells. Peptide regurgitation was not involved. Since particulate, nonreplicating antigens can efficiently prime anti-viral cytotoxic T cells in vivo, this new, TAP-independent pathway of class I-associated antigen presentation may be applicable for vaccine strategies.