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Tannins elevate the level of poly(ADP–ribose) in HeLa cell extracts

✍ Scribed by Claudia Keil; Eva Petermann; Shiao Li Oei


Book ID
116176957
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
289 KB
Volume
425
Category
Article
ISSN
0003-9861

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ADP-ribose polymers were isolated from living mammalian cells, separated by polyacrylamide gel electrophoresis, visualized in the gel with a novel silver staining agent, and quantified by computer-aided scanning densitometry. This method detects as little as \(\sim 40 \mathrm{fmol}\) of ADP-ribose p