Poly(ADP-ribose) Quantification at the Femtomole Level in Mammalian Cells

✍ Scribed by M. Malanga; S. Bachmann; P.L. Panzeter; B. Zweifel; F.R. Althaus

• Book ID: 102968668
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 655 KB
• Volume: 228
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1346

✦ Synopsis

ADP-ribose polymers were isolated from living mammalian cells, separated by polyacrylamide gel electrophoresis, visualized in the gel with a novel silver staining agent, and quantified by computer-aided scanning densitometry. This method detects as little as (\sim 40 \mathrm{fmol}) of ADP-ribose polymers of a particular size class and reduces the gel exposure times required for conventionally radiolabeled polymers from 2 months to about (1 \mathrm{~h}). The method also detects polymers with slow turnover which may be underestimated by techniques requiring metabolic radiolabeling of poly(ADP-ribose). c 1995 Academic Press, Inc.