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Tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase

✍ Scribed by Moon-Jung Kim; Byung Cheon Lee; Jaeho Jeong; Kong-Joo Lee; Kwang Yeon Hwang; Vadim N. Gladyshev; Hwa-Young Kim


Book ID
110836856
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
784 KB
Volume
79
Category
Article
ISSN
0950-382X

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Reduction of N-acetyl methionine sulfoxi
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A simple and rapid assay has been developed to measure the enzymatic activity of peptide methionine sulfoxide reductase. The assay is based on the reduction of labeled N-acetylmethionine sulfoxide to N-acetvlmethionine. The N-acetylmethionine can be separated from the substrate by extraction into et