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Tandem mass spectrometry of peptides. Relationship between translational energy loss and fragment ion mass

✍ Scribed by Margaret M. Sheil; Peter J. Derrick

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
734 KB
Volume
23
Category
Article
ISSN
1076-5174

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✦ Synopsis

Incident ion energy

Ei and collision gas pressure have been adjusted so as to obtain a satisfactory tandem mass spectrum of the m/z 1882 ion formed from valine-gramicidin A (relative molecular mass 1881). Translational energy losses AE have been determined (at Ei = 14.8 keV) from the precise positions of a large number of fragment ion peaks in the spectrum. The variation in AE for different fragment ions is great and the magnitudes of AE are large (being commonly of the order of 10' eV). It is shown how very large energy losses AE can arise, if the parent ion decomposes to a small fragment ion which itself collides and decomposes further. Implications of the dependence of AE upon fragment ion mass for the scan laws of four-sector instruments are discussed briefly.

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