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Tandem mass spectrometry of peptides. III—differentiation between leucine and isoleucine based on neutral losses

✍ Scribed by Nicole L. Squire; Šárka Beranová; Chrys Wesdemiotis

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
550 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The neutral species generated during the collisionally activated dissociation (CAD) of the protonated dipeptides Ala–Leu, Ala–Ile, Leu–Ala and Ile–Ala are post‐ionized by collision and detected in neutral fragment‐reionization (^+^N~f~R^+^) mass spectra. These spectra are dominated by fragment ions diagnostic for the C‐terminal amino acid, as confirmed by comparison with reference collision‐induced dissociative ionization (CIDI) mass spectra of authentic Ala, Leu and Ile. CIDI of Leu and Ile yields distinct and structurally indicative products. These are also observed upon ^+^N~f~R^+^ of [Ala–Leu]H^+^ and [Ala–Ile]H^+^, respectively, thus allowing one to deduce the correct sequence. In contrast, [Leu–Ala]H^+^ and [Ile–Ala]H^+^, which possess identical C‐termini, form no differentiating neutral losses upon CAD.

📜 SIMILAR VOLUMES

Tandem mass spectrometry of peptides: Se
Tandem mass spectrometry of peptides: Sequence information based on neutral losses. I—isomeric dipeptides
✍ Marcela M. Cordero; Chrys Wesdemiotis 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB

## Abstract The neutral losses arising on the collisionally activated dissociation (CAD) of protonated dipeptides (MH^+^) are reionized by collision and detected in neutral fragment‐reionization (N~f~R) mass spectra. For the dipeptides investigated, which encompass Ala–Gly/Gly–Ala, Asp–Gly/Gly–Asp,

Tandem mass spectrometry of peptides: Me
Tandem mass spectrometry of peptides: Mechanistic aspects and structural information based on neutral losses. II—Tri- and larger peptides
✍ Marcela M. Cordero; Chrys Wesdemiotis 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 717 KB

## Abstract The neutral products arising during the collisionally activated dissociation of protonated oligopeptides (MH^+^) are post‐ionized by collision and detected in neutral fragment‐reionization (^+^N~f~R^+^) mass spectra. For the isomeric tripeptides Ala‐Gly‐Gly, Gly‐Ala‐Gly and Gly‐Gly‐Ala,