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Tailored Correlation Spectroscopy for the Enhancement of Fingerprint Cross Peaks in Peptides and Proteins

✍ Scribed by J. Quant; T. Prasch; S. Ihringer; S.J. Glaser

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
496 KB
Volume
106
Category
Article
ISSN
1064-1866

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✦ Synopsis

A new experimental mixing scheme for band-selective Hartmann-Hahn transfer between the (\mathrm{H}^{\mathrm{N}}) and (\mathrm{H}{\alpha}) resonances of peptides and proteins is presented. This form of tailored correlation spectroscopy (TACSY) allows enhancement of the sensitivity of specific ( (\mathrm{H}^{\mathrm{N}}-\mathrm{H}{\alpha}) )-fingerprint signals compared to broadband TOCSY experiments. The design criteria as well as experimental applications of the multiple-pulse sequence CABBY-1 are presented. c 1995 Academic Press, Inc.

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