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t(2;8) variant translocation in burkitt's lymphoma: Mapping of chromosomal breakpoints by in situ hybridization

✍ Scribed by Sabine Adolph; Horst Hameister; Berthold Henglein; Martin Lipp; Peter Hartl; Frank Baas; Gilbert Lenoir; Georg W. Bornkamm

Publisher: John Wiley and Sons
Year: 1989
Tongue: French
Weight: 719 KB
Volume: 44
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910440213

No coin nor oath required. For personal study only.

✦ Synopsis

In 6 different Burkitt lymphoma cell lines with t(2;8) variant translocations (JI, LY66, LY91, B U I , BL64, JBU) the breakpoints on chromosome 8q+ were mapped in relation to each other and to c-myc by in situ hybridization. The probes used were derived from chromosome 8q24 and comprised a c-myc probe, a probe located 48 kb downstream of c-myc, 3 probes adjacent to the chromosomal breakpoints of BL64, LY9l and JBU, respectively, and 2 probes located in the 5' and 3' part of the thyroglobulin gene. The breakpoints of LY9l and J B U lie ZOO kb downstream of c-myc, whereas the distance to c-myc of the BL64 breakpoint and of the thyroglobulin probes is unknown. By recording the hybridization signals specific for these probes on chromosomes Zp-and 8q+ of each cell line it was possible to establish the order of breakpoints on band 8q24 relative to the c-myc and thyroglobulin genes as follows: centromere-c-my~Jl-BL64-BLZl-LY9 I-JBLZ-LY66thyroglobulin-telomere.
This information is essential for further mapping of this important chromosomal region.

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